RT Journal Article SR Electronic A1 Puchnin, Maxim A1 Pešlová, Františka A1 Kuchař, Jiří T1 Optimization of Titanium Surface for Live Cells JF Manufacturing Technology Journal YR 2025 VO 25 IS 1 SP 103 OP 112 DO 10.21062/mft.2025.010 UL https://journalmt.com/artkey/mft-202501-0009.php AB The present paper is focused on the study of the characteristics of selected titanium alloys before and after heat treatment. The specimens were cooled both in water and liquid nitrogen from 900°C and 1000°C for pure titanium and from 1000°C and 1100°C for the Ti-6Al-4V alloy. Further, the paper deals with line MG63 live bone cells deposited on a titanium base substrate. Proliferation and differentiation are monitored of cells during 7-day in vitro cultivation portraying growth of cells on a biologically selected material.