Manufacturing Technology 2018, 18(5):866-872 | DOI: 10.21062/ujep/192.2018/a/1213-2489/MT/18/5/866

Uniaxial Tensile Testing Device for Measuring Mechanical Properties of Biological Tissue with Stress-Relaxation Test under a Confocal Microscope

David Vondrášek1,2, Daniel Hadraba1,2, Roman Matějka2, František Lopot1, Martin Svoboda3, Karel Jelen1
1 Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic
2 Institute of Physiology, Czech Academy, Prague, Czech Republic
3 Faculty of Mechanical Engineering, Jan Evangelista Purkyně University in Ústí nad Labem, Pasteurova 1, Ústí nad Labem, Czech Republic

Biological soft tissue is a non-linear and viscoelastic material and its mechanical properties can greatly affect quality of life. Many external mechanical factors can alter the tissue, for example the tissue of talipes equinovarus congenitus, also known as clubfoot, which is the most frequent congenital deformity affecting lower extremities with pathological changes of connective tissue. In clubfoot, the presence of disc-like mass of fibrous tissue, resembling intervertebral disc tissue, is described to be between the medial malleolus and the medial side of the navicular bone. The clubfoot tissue is often referred to be stiffer or rigid by clinicians, or it is referred to as contracted and less contracted tissue, however relevant evidence about mechanical properties is missing. Therefore, the description "disc-like" is informing only about relative mechanical properties of clubfoot tissue. We aim to prepare methodical approach to quantify mechanical properties of biological tissue with uniaxial tensile stress-relaxation test, in order to help clinicians and scientist to identify precisely the mechanical properties of normal and pathological tissue and their structural behaviour during mechanical testing. In this study, we test and tune the uniaxial tensile stress-relaxation test on biological tissue with high content of connective tissue such as collagen. The model tissue is porcine pericardium. The tissue has clear collagen fibres aligning parallel to the force applied. Modulus of elasticity measured here is comparable to other studies.

Keywords: uniaxial tensile test, stress-relaxation test, biomechanical models, Maxwell-Wiechert's model, talipes equinovarus congenitus, porcine pericardium, mechanical properties of biological tissues, fibroproliferative diseases

Published: October 1, 2018  Show citation

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Vondrášek D, Hadraba D, Matějka R, Lopot F, Svoboda M, Jelen K. Uniaxial Tensile Testing Device for Measuring Mechanical Properties of Biological Tissue with Stress-Relaxation Test under a Confocal Microscope. Manufacturing Technology. 2018;18(5):866-872. doi: 10.21062/ujep/192.2018/a/1213-2489/MT/18/5/866.
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